An NMD Pathway in Yeast Involving Accelerated Deadenylation and Exosome-Mediated 3′→5′ Degradation

CYP3A5 mRNA degradation by nonsense mediated mRNA decay (NMD)

Rapid deadenylation triggered by a nonsense codon precedes decay of the RNA body in a mammalian cytoplasmic nonsense-mediated decay pathway.

Nonsense-mediated mRNA decay (NMD) is an RNA surveillance pathway that detects and destroys aberrant mRNAs containing nonsense or premature termination codons (PTCs) in a translation-dependent manner in eukaryotes. In yeast, the NMD pathway bypasses the deadenylation step and directly targets PTC-containing messages for decapping, followed by 5'-to-3' exonuclease digestion of the RNA body. In m...

An mRNA surveillance mechanism that eliminates transcripts lacking termination codons.

Translation is an important mechanism to monitor the quality of messenger RNAs (mRNAs), as exemplified by the translation-dependent recognition and degradation of transcripts harboring premature termination codons (PTCs) by the nonsense-mediated mRNA decay (NMD) pathway. We demonstrate in yeast that mRNAs lacking all termination codons are as labile as nonsense transcripts. Decay of "nonstop" t...

Exonuclease hDIS3L2 specifies an exosome-independent 3'-5' degradation pathway of human cytoplasmic mRNA.

Turnover of mRNA in the cytoplasm of human cells is thought to be redundantly conducted by the monomeric 5'-3' exoribonuclease hXRN1 and the 3'-5' exoribonucleolytic RNA exosome complex. However, in addition to the exosome-associated 3'-5' exonucleases hDIS3 and hDIS3L, the human genome encodes another RNase II/R domain protein-hDIS3L2. Here, we show that hDIS3L2 is an exosome-independent cytop...

Autism and Nonsense Mediated Decay (NMD)

In 2010 large scale screening study of a number of families with neurodevelopmental disorders including autism, a family from Belgium with two brothers and one brother was presented with ID and autism; it was found that he has a single nucleotide substitution in UPF3b 1103G>A forming Arg368Gln (R368Q). The mother of the boy was found to be a carrier too. Interestingly, the missense mutation in ...